OBJECTIVE: The purpose of this investigation is to determine if the protein expression of the Wnt/β-catenin signal pathway is induced after acute renal injury in patients with acute tubular necrosis.
PATIENTS AND METHODS: Sixty patients in who met inclusion criteria underwent renal biopsy. Based on the result of the biopsy, patients were divided into two groups: one with nephrotic syndrome with mini-change disease (MCD group, 30 patients), the other one with minor lesions and acute tubular necrosis (ATN, 30 patients). Biopsy sections were stained with either periodic acid-Schiff (PAS) or Masson stain and examined under light microscopy to determine the degree of tubulointerstitial injury and renal tubular interstitial fibrosis, respectively. The expression of Wnt/β-catenin signal proteins in renal tissue was resolved immunohistochemically using protein-specific antisera.
RESULTS: The typically silent Wnt/β-catenin signal pathway in normal kidneys was noted to be upregulated in patients in either the MCD and ATN groups; that expression was statistically significantly higher in the ATN group as compared to the MCD group (p < 0.05). The degree of renal tissue injury was statistically significantly higher in the ATN group as compared to the MCD group (p < 0.05). Also, the degree of tubulointerstitial fibrosis was significantly higher in the ATN group as compared to the MCD group (p < 0.05).
CONCLUSIONS: Results of this investigation confirm the expression of Wnt/β-catenin signal pathway in renal tissue in patients with acute renal injury and nephrotic syndrome, which appear to be important in the host biological response to necrosis, fibrosis, and proliferation.Free PDF Download
To cite this article
S.-Y. Wang, S.-X. Yang, X.-X. Zhao, F. Chen, J. Shi
Expression of the Wnt/β-catenin signal pathway in patients with acute renal injury
Eur Rev Med Pharmacol Sci
Vol. 21 - N. 20