Eur Rev Med Pharmacol Sci 2017; 21 (24): 5789-5797

DOI: 10.26355/eurrev_201712_14026

The mechanism of miR-23a in regulating myocardial cell apoptosis through targeting FoxO3

Q.-H. Yang, M. Yang, L.-L. Zhang, M.-C. Xiao, Y. Zhao, D.-X. Yan

Department of Cardiology, The Fourth Affiliated Hospital of Harbin Medical University, Heilongjiang, China. lilizhang564@163.com


OBJECTIVE: Myocardial cell apoptosis represents important pathologic basis of ischemia-reperfusion injury (I/R). MiR-23a is related to myocardial hypertrophy and cardiac remodeling by regulating myocardial cell growth and apoptosis. This study intended to observe the regulating effect of miR-23a in myocardial cell and related target, and investigate its clinical significance to I/R injury.

MATERIALS AND METHODS: The rats were divided into sham group and myocardial I/R group. Myocardial cell cycle and miR-23a expression were tested. H2O2 was applied to treat H9c2 rat myocardial cell to simulate oxidative stress during I/R. The cells were divided into blank group, NC group, miR-23a mimic group, H2O2 group, and miR-23a + H2O2 group. ROS content and cell apoptosis were detected by flow cytometry. MiR-23a, FoxO3a, and BIM gene expression were determined by qRT-PCR. FoxO3a and BIM protein levels were measured by Western blot.

RESULTS: Compared with sham group, myocardial apoptosis increased, while miR-23a expression was significantly downregulated in I/R group. H2O2 treatment markedly increased ROS levels in H9c2 cells and elevated apoptosis. The overexpression of mMiR-23a effectively reduced cell apoptosis induced by H2O2 treatment. H2O2 treatment significantly decreased miR-23a expression, while markedly elevated the levels of FoxO3a and BIM. The overexpression of miR-23a apparently impeded the induction of FoxO3a and BIM by H2O2.

CONCLUSIONS: The downregulation of miR-23a plays a negative role in oxidative stress and cell apoptosis induced by I/R. The overexpression of miR-23a is of significance to alleviate cell apoptosis through inhibiting FoxO3a and downstream target BIM expression.

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To cite this article

Q.-H. Yang, M. Yang, L.-L. Zhang, M.-C. Xiao, Y. Zhao, D.-X. Yan
The mechanism of miR-23a in regulating myocardial cell apoptosis through targeting FoxO3

Eur Rev Med Pharmacol Sci
Year: 2017
Vol. 21 - N. 24
Pages: 5789-5797
DOI: 10.26355/eurrev_201712_14026