OBJECTIVE: To study the link between hepatic interleukin-6 (IL-6) and osteopontin (OPN) gene expression and vitamin D3 status associated with type 1 diabetes in mice; and to evaluate the effects of vitamin D3 treatment (800 IU/kg of body weight for 6 weeks) on diabetes-induced impairments.
MATERIALS AND METHODS: mRNA levels of IL-6 and OPN were measured by quantitative RT-PCR. Blood serum 25OHD3 was assayed by ELISA.
RESULTS: It was shown that induction of IL-6 in diabetic liver is accompanied by increased expression of OPN. Changes in OPN and IL-6 RNA levels correlated with a lack of 25OHD3 in serum. Vitamin D3 treatment restored 25OHD3 that led to a substantial reduction of OPN and IL-6 mRNA levels.
CONCLUSIONS: Diabetes-induced vitamin D3 deficiency was associated with increased hepatic levels of IL-6 and OPN mRNA and these changes were countered by vitamin D3 administration.Free PDF Download
To cite this article
D. Labudzynskyi, I. Shymanskyy, M. Veliky
Role of vitamin D3 in regulation of interleukin-6 and osteopontin expression in liver of diabetic mice
Eur Rev Med Pharmacol Sci
Vol. 20 - N. 13