OBJECTIVE: Lung cancer seriously threats to patient’s life and health. Cyramza is a therapeutic drug for inhibition of vessel formation and growth in clinical practice. The aim of this study was to investigate the effect of cyramza on growth and apoptosis of non-small lung cancer HCC4006 and explore the related mechanisms.
MATERIALS AND METHODS: Cell viability and apoptosis were examined by MTT assay and flow cytometry, respectively. Western blot was employed to examine the effect of cyramza on the apoptotic protein Bcl-w. After that Bcl-w knockdown and overexpression were respectively fulfilled by Bcl-w siRNA and plasmid transfection, effects of cyramza on cell apoptosis were determined by Western-blot.
RESULTS: Cyramza inhibited the cell growth and induced the cell apoptosis in HCC4006 cells, which was mediated by downregulation of Bcl-w level. Bcl-w knockdown and overexpression could increase and decrease the effect of cyramza on cell apoptosis, respectively.
CONCLUSIONS: Cyramza induced the apoptosis of non-small lung cancer cell line HCC4006 via the downregulation of Bcl-w.Free PDF Download
To cite this article
L. Fang, Z. Li, Y.-J. Chen, G.-M. Xiao
Cyramza induces apoptosis of HCC4006 cell by affecting the level of Bcl-w
Eur Rev Med Pharmacol Sci
Vol. 21 - N. 13