OBJECTIVE: To dissect the functioning mode of miR-582-5p on gastric cancer cell growth and provide therapeutic targets for gastric cancer.
PATIENTS AND METHODS: Relative expression levels of miR-582-5p in human gastric cancer tissue samples and gastric cancer-derived cell lines were measured by using quantitative Real-time PCR. Cell proliferation and viability capacities were assessed by cell counting kit-8 (CCK8) assay and colony formation assay. Cell apoptosis and cell cycle distribution were identified by flow cytometry. Downstream target gene was confirmed by using luciferase and Western blotting assays.
RESULTS: MiR-582-5p was downregulated in gastric cancer tissues when compared with para-carcinoma tissues (n=42). Overexpressed miR-582-5p could attenuate cell proliferation and viability capacities, as well as promoted cell apoptosis and cell cycle arrest at G0/G1 phase. AKT3 was chosen as the target gene of miR-582-5p by bioinformatics analysis and luciferase reporter assay. Moreover, restoration of AKT3 could impair tumor suppression role of miR-582-5p on gastric cancer growth.
CONCLUSIONS: MiR-582-5p exerted tumor-suppressive effects on gastric cancer growth via targeting AKT3 in vitro, which provided an innovative and candidate target for diagnosis and treatment of gastric cancer.Free PDF Download
To cite this article
Y. Jin, L.-P. Tao, S.-C. Yao, Q.-K. Huang, Z.-F. Chen, Y.-J. Sun, S.-Q. Jin
MicroRNA-582-5p suppressed gastric cancer cell proliferation via targeting AKT3
Eur Rev Med Pharmacol Sci
Vol. 21 - N. 22