Eur Rev Med Pharmacol Sci 2018; 22 (6): 1549-1553

DOI: 10.26355/eurrev_201803_14559

Effects of testosterone on PPARγ and P450arom expression in polycystic ovary syndrome patients and related mechanisms

Y. He, C.-L. Wang

Department of Obstetrics, Jinan Maternity and Child Care Hospital, Jinan, Shandong, China. 3370507921@qq.com


OBJECTIVE: Polycystic ovary syndrome (PCOS) causes ovulation disorder and infertility in female patients. This study recruited PCOS patients and adopted testosterone therapy to analyze its effect on expression of nuclear peroxisome proliferation activated receptor γ (PPARγ), and cytochrome P450 aromatase (P450arom) in ovary granular cells of patients.

PATIENTS AND METHODS: PCOS and non-PCOS patients were treated with testosterone at 1.0 nmol/L, 10 nmol/L. qRT-PCR was used to detect the expressions of PPARγ and P450arom while Western blot was performed for the evaluation of protein expression.

RESULTS: In the experimental group, the level of PPARγ mRNA was significantly elevated whilst the expression of P450arom was statistically decreased (p < 0.05). The treatment of 10 nmol/l testosterone significantly elevated PPARγ mRNA and protein expression, and decreased P450arom expression (p < 0.05). The level of PPARγ was positively correlated with testosterone concentration, and was negatively correlated with P450arom expression (p < 0.05).

CONCLUSIONS: Testosterone causes hyperandrogenism microenvironment of PCOS patients, with a correlated increase of PPARγ and reduction of P450arom.

Free PDF Download

To cite this article

Y. He, C.-L. Wang
Effects of testosterone on PPARγ and P450arom expression in polycystic ovary syndrome patients and related mechanisms

Eur Rev Med Pharmacol Sci
Year: 2018
Vol. 22 - N. 6
Pages: 1549-1553
DOI: 10.26355/eurrev_201803_14559