Eur Rev Med Pharmacol Sci 2018; 22 (9): 2824-2831

DOI: 10.26355/eurrev_201805_14983

LncRNA MEG3 participates in neuronal cell injury induced by subarachnoid hemorrhage via inhibiting the Pi3k/Akt pathway

Z. Liang, Y.-J. Chi, G.-Q. Lin, L.-F. Xiao, G.-L. Su, L.-M. Yang

Department of Neurosurgery, Lianjiang Hospital Affiliated To Guangdong Medical University/Lianjiang People’s Hospital, Lianjiang, China. liangzaizai008@163.com


OBJECTIVE: To explore the effect of LncRNA MEG3 in the subarachnoid hemorrhage (SAH) and its underlying mechanism.

PATIENTS AND METHODS: The expressions of lncRNA MEG3 in SAH patients and animal model were detected by quantitative real-time PCR (qRT-PCR). After LncRNA MEG3 was overexpressed in neurons by lentivirus, viability and apoptosis abilities were detected by cell counting kit-8 (CCK-8) assay, flow cytometry, and TUNEL assay, respectively. The apoptosis-related genes and Pi3k/Akt pathway-related proteins were further detected by a Western blot.

RESULTS: The expressions of lncRNA MEG3 in SAH patients were remarkably higher than normal controls, which were positively correlated with SAH severity. After lncRNA MEG3 overexpression, neuronal cell activity was decreased and cell apoptosis was increased. Moreover, the expressions of Bax, p53, and cleaved Caspase-3 were increased, whereas the expression of Bcl-2 and Pi3k/Akt pathway-related proteins were decreased after lncRNA MEG3 overexpression.

CONCLUSIONS: LncRNA MEG3 is up-regulated in SAH, which may promote SAH-induced neuronal cell injury via inhibition of the Pi3k/Akt pathway.

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To cite this article

Z. Liang, Y.-J. Chi, G.-Q. Lin, L.-F. Xiao, G.-L. Su, L.-M. Yang
LncRNA MEG3 participates in neuronal cell injury induced by subarachnoid hemorrhage via inhibiting the Pi3k/Akt pathway

Eur Rev Med Pharmacol Sci
Year: 2018
Vol. 22 - N. 9
Pages: 2824-2831
DOI: 10.26355/eurrev_201805_14983