Eur Rev Med Pharmacol Sci 2018; 22 (9): 2876-2887

DOI: 10.26355/eurrev_201805_14990

Combinatory effect of mesenchymal stromal cells transplantation and quercetin after spinal cord injury in rat

X. Wang, Y.-Y. Wang, L.-L. Zhang, G.-T. Li, H.-T. Zhang

Department of Neurosurgery, The People’s Hospital of Rizhao, Shandong, China. zhanghongtian007@126.com


OBJECTIVE: To investigate the synergistic effects of quercetin (Qu) administration and transplantation of human umbilical cord mesenchymal stromal cells (HUMSCs) following spinal cord injury (SCI).

MATERIALS AND METHODS: HUMSCs were isolated, cultured and certificated via flow cytometry. Sixty Sprague-Dawley (SD) female rats were used and SCI models were made. All rats were divided into five experimental groups: culture medium treated group (n=28); HUSMCs + quercetin-treated group (n = 28); HUMSCs treated group (n=28); quercetin-treated group (n = 28); sham group (n = 20). Basso, Beattie, and Bresnahan (BBB) were used to assess neurological function recovery. Axons at the injury epicenter of the injury were checked by immunohistochemical analysis. Cystic cavity was measured and rat cytokine Luminex custom 8-plex kits (for interleukin (IL)-4, IL-1β, IL-6, IL-10, interferon (IFN)-γ, tumor necrosis factor (TNF)-β1) were checked.

RESULTS: The combination treatment with Qu and delayed transplantation of HUMSCs after rat SCI improved neurological functional recovery, increased axonal preservation, promoted macrophage polarization, decreased the size of the cystic cavity, reduced the proinflammatory cytokines, including IL-1β and IL-6. Also, it increased anti-inflammatory cytokines, including IL-4, IL-10, and transforming growth factor (TGF)-β1.

CONCLUSIONS: We showed that HUMSCs transplantation in combination with Qu was a potential strategy for reducing secondary damage and promoting functional recovery following SCI.

 

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To cite this article

X. Wang, Y.-Y. Wang, L.-L. Zhang, G.-T. Li, H.-T. Zhang
Combinatory effect of mesenchymal stromal cells transplantation and quercetin after spinal cord injury in rat

Eur Rev Med Pharmacol Sci
Year: 2018
Vol. 22 - N. 9
Pages: 2876-2887
DOI: 10.26355/eurrev_201805_14990