Eur Rev Med Pharmacol Sci 2018; 22 (13): 4156-4165

DOI: 10.26355/eurrev_201807_15408

MiR-550a-3p promotes non-small cell lung cancer cell proliferation and metastasis through down-regulating TIMP2

J.-Z. Yang, L. Bian, J.-G. Hou, H.-Y. Wang

Department of Radiation Oncology, The Second Hospital of Jilin University, Changchun, China. 27382909@qq.com


OBJECTIVE: MicroRNAs (miRNAs) have been identified to play a crucial regulatory role in the development and progression of malignant tumors, including lung cancer. However, the function of miR-550a-3p on the progression of non-small cell lung cancer (NSCLC) remains poorly understood.

PATIENTS AND METHODS: Quantitative Real-time polymerase chain reaction was employed to estimate the expression level of miR-550a-3p in NSCLC tissue and cell samples. Cell proliferation was measured by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) and colony formation assays. Transwell assay was recruited to demonstrate the abilities of cell invasion and migration. Luciferase analysis and Western-blot assay were performed to elucidate the underlying mechanism of miR-550a-3p in NSCLC.

RESULTS: The level of miR-550a-3p expressed in NSCLC tissues was significantly higher than that in para-tumor control tissues. Over-expression of miR-550a-3p significantly promoted proliferation, invasion, and migration of A549 cells while knockdown of miR-550a-3p inhibited growth and metastasis of H460 cells. TIMP2 was verified as a direct target of miR-550a-3p in NSCLC. Restoration of TIMP2 rescued the influence of miR-550a-3p over-expression.

CONCLUSIONS: We demonstrated that miR-550a-3p regulated the progression of NSCLC cells through TIMP2. Thus, miR-550a-3p axis could serve as a potential therapeutic target for NSCLC treatment.

Free PDF Download

To cite this article

J.-Z. Yang, L. Bian, J.-G. Hou, H.-Y. Wang
MiR-550a-3p promotes non-small cell lung cancer cell proliferation and metastasis through down-regulating TIMP2

Eur Rev Med Pharmacol Sci
Year: 2018
Vol. 22 - N. 13
Pages: 4156-4165
DOI: 10.26355/eurrev_201807_15408