OBJECTIVE: To establish a mouse lymphoma xenograft model so as to investigate the correlation between the expression of matrix metallopeptidase-9 (MMP-9) and that of B-cell lymphoma 2 (Bcl-2) in lymphomas.
MATERIALS AND METHODS: Diffuse large Bcl (DLBCL) cells were cultured, and a mouse lymphoma xenograft model was established via the subcutaneous injection. Mouse lymphoma tissues were extracted, and the expressions of MMP-9 and Bcl-2 messenger ribonucleic acids (mRNAs) in the xenograft tumor were detected using Real-time polymerase chain reaction (PCR). Immunohistochemistry was used to detect the expression levels of MMP-9 and Bcl-2 proteins in lymphoma tissues and tumor-adjacent tissues. The consistency of MMP-9 expression and Bcl-2 expression was analyzed via Spearman’s rank correlation analysis.
RESULTS: The expressions of MMP-9 and Bcl-2 in lymphoma tissues were increased. The expression levels of MMP-9 and Bcl-2 proteins in lymphoma tissues were higher than those in tumor-adjacent tissues. The expression levels of MMP-9 and Bcl-2 were correlated with the body weight loss degree of mice, and the expression of MMP-9 was positively associated with that of BCL-2 in lymphomas.
CONCLUSIONS: MMP-9 and Bcl-2 are associated with the onset of DLBCL, and they are potential impact factors affecting the prognosis.Free PDF Download
To cite this article
C.-L. Shi, X.-Y. Zhang, Y. Li, L.-L. Song, L. Wang
Correlations of mouse lymphoma xenografts with the expressions of MMP-9 and Bcl-2
Eur Rev Med Pharmacol Sci
Vol. 23 - N. 3