OBJECTIVE: To investigate the effect of miR-214-5p on spinal cord injury (SCI) and the possible mechanism in pathophysiological relevance, and to evaluate the therapeutic efficacy of the corresponding inhibitor.
MATERIALS AND METHODS: The SCI model was successfully established in 6-week-old rats. The levels of locomotor function recovery in rats of miR-214-5p inhibitor group and SCI group were detected one month later by Basso-Beattie-Bresnahan (BBB) locomotor rating scale, Western blotting, quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Rat microglia cells were cultured in vitro. Furthermore, the effect of miR-214-5p and its inhibitor on inflammatory microglia was explored.
RESULTS: Compared with SCI group, rats in miR-214-5p inhibitor group showed a significant retardation of inflammatory diffusion in terms of reduced production of inflammatory factors and chemokines in vivo. MiR-214-5p inhibitor markedly attenuated antioxidant stress, inhibited apoptosis, and increased nerve fibers repair. Compared with lipopolysaccharide (LPS) group, tumor necrosis factor alpha (TNF-α) and interleukin 1 (IL-1) decreased significantly in microglia treated with miR-214-5p inhibitor in vitro. Furthermore, inhibition of miR-214-5p remarkably promoted locomotor function recovery in rats.
CONCLUSIONS: MiR-214-5p inhibitor retarded inflammatory diffusion by inhibiting inflammatory factors and chemokines after SCI. In addition, this might relieve nerve structure destruction, resist oxidative stress and inhibit apoptosis, eventually promoting function recovery.Free PDF Download
To cite this article
P. Wang, Z.-W. Li, Z. Zhu, Z.-Y. Zhang, J. Liu
Inhibition of miR-214-5p attenuates inflammatory chemotaxis and nerve regeneration obstruction after spinal cord injury in rats
Eur Rev Med Pharmacol Sci
Vol. 23 - N. 6