OBJECTIVE: Our study aims to explore whether microRNA-940 could participate in early abortion by inhibiting placenta implantation and its underlying mechanism.
PATIENTS AND METHODS: Expressions of microRNA-940 and ZNF672 in the placental villi of 6 early abortion pregnancies and 6 normal pregnancies were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Expressions of microRNA-940 and ZNF672 in trophoblast cells (BeWo, JEG3, Wish and HTR-8) were also detected. Cell counting kit-8 (CCK-8) assay was performed to detect the effect of microRNA-940 on the proliferation of trophoblast cells after being transfected with microRNA-940 inhibitor or mimics, respectively. Dual-Luciferase reporter gene assay and RNA binding protein immunoprecipitation (RIP) assay were conducted to demonstrate the binding condition of microRNA-940 to ZNF672.
RESULTS: MicroRNA-940 was highly expressed in placental villi of early abortion pregnancies, whereas ZNF672 was lowly expressed. HTR-8 cells expressed the highest level and BeWo cells expressed the lowest level of microRNA-940. After transfection of microRNA-940 inhibitor in HTR-8 cells, the proliferative capacity was remarkably increased. On the contrary, the transfection of microRNA-940 mimic downregulated the proliferation of BeWo cells. Dual-Luciferase reporter gene assay demonstrated that microRNA-940 targets ZNF672. RIP results further indicated that microRNA-940 binds to ZNF672.
CONCLUSIONS: MicroRNA-940 is highly expressed in the placental villi of early abortion pregnancies and promotes the occurrence of early abortion by inhibiting the proliferation of trophoblast cells by targeting ZNF672.
To cite this article
C.-Y. Zhang, X.-L. Lu, Y. Yang, N. Ayi, L.-H. Li
Highly expressed microRNA-940 promotes early abortion by regulating placenta implantation by targeting ZNF672
Eur Rev Med Pharmacol Sci
Vol. 23 - N. 7