Up-regulated circular RNA hsa_circ_0067934 contributes to glioblastoma progression through activating PI3K-AKT pathway

J. Xin, X.-Y. Zhang, D.-K. Sun, L.-Q. Tian, P. Xu

Department of Neurosurgery, Linyi Center Hospital, Linyi, Shandong, China. okppqq2234@163.com

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• Oncology

OBJECTIVE: Circular RNAs (circRNAs) are involved in cancer progression and metastasis, although little is known about their role in glioblastoma (GBM). The aim of this study was to investigate the expression pattern and molecular mechanism of hsa_circ_0067934 in GBM.
PATIENTS AND METHODS: Hsa_circ_0067934 expression was detected in GBM tissues and cell lines by using RT-PCR. The prognostic value of the hsa_circ_0067934 expression in GBM patients was statistically analyzed. The effects of hsa_circ_0067934 expression on growth and apoptosis of GBM cells were analyzed by CCK-8, colony formation, and flow cytometry. Migration and invasion ability was evaluated by transwell and wound-healing assays. The protein expression of signaling pathways was determined by using Western blot.
RESULTS: The expression level of hsa_circ_0067934 was significantly increased in GBM tissues and cancer cells. Kaplan-Meier survival curves showed that patients with higher expression of hsa_circ_0067934 had a more favorable overall survival and disease-free survival. The functional investigations revealed that the knockdown of hsa_circ_0067934 suppressed GBM cell proliferation, metastasis and EMT, and promoted apoptosis. Moreover, the mechanistic analysis demonstrated that the down-regulation of hsa_circ_0067934 significantly suppressed the activation of the PI3K-AKT pathway.
CONCLUSIONS: Hsa_circ_0067934 is overexpressed and plays an oncogenic role in GBM by promoting cancer cell proliferation and metastasis via upregulation of PI3K-AKT pathway, which suggests that hsa_circ_0067934 is likely to serve as an efficient therapeutic approach in respect of GBM treatment.

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