Eur Rev Med Pharmacol Sci 2019; 23 (9): 3806-3812

DOI: 10.26355/eurrev_201905_17807

MicroRNA-142 promotes the development of nasopharyngeal carcinoma through targeting PTEN

D.-P. Li, W. Chai, Y.-H. Liu, T.-T. Xu, H. Huang

Department of Otorhinolaryngology Head and Neck Surgery, The People’s Hospital of Bozhou; Bozhou Clinical Medicine College of Anhui Medical University; Affiliated Bozhou Hospital of Anhui University of Science and Technology, Bozhou, China


OBJECTIVE: To elucidate whether microRNA-142 could regulate the development of nasopharyngeal carcinoma (NPC) by mediating gene of phosphate and tension homology deleted on chromosome ten (PTEN) expression.
PATIENTS AND METHODS: The microRNA-142 expression in NPC tissues and paracancerous tissues was detected by the quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Correlation between the microRNA-142 expression and the prognosis of NPC patients was analyzed. MicroRNA-142 expression in NPC cell lines was determined as well. By transfection of microRNA-142 inhibitor or negative control, biological performances of NPC cells were accessed through cell counting kit-8 (CCK-8), colony formation, wound healing, and transwell assay. Dual-luciferase reporter gene assay was conducted to verify the binding condition between microRNA-142 and its target gene PTEN. Rescue experiments were carried out by co-transfection of microRNA-142 inhibitor and si-PTEN, followed by detecting the invasive capacity of NPC cells. Protein expressions of relative genes in the PI3K/AKT pathway after the microRNA-142 knockdown in NPC cells were determined by Western blot.
RESULTS: MicroRNA-142 was highly expressed in NPC tissues than that of paracancerous tissues, which was correlated with poor prognosis of NPC patients. MicroRNA-142 was also highly expressed in NPC cells. Downregulated microRNA-142 inhibited proliferative, migratory, and invasive capacities of NPC cells. Dual-luciferase reporter gene assay verified that microRNA-142 could directly bind to PTEN. Knockdown of PTEN could reverse the inhibitory effect of microRNA-142 on invasive capacity of NPC cells. Finally, Western blot results demonstrated that the microRNA-142 knockdown inhibited the PI3K/AKT pathway in NPC cells.
CONCLUSIONS: MicroRNA-142 is highly expressed in NPC. MicroRNA-142 enhances the proliferative and invasive capacities of NPC cells by inhibiting PTEN expression, thus promoting NPC development.

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To cite this article

D.-P. Li, W. Chai, Y.-H. Liu, T.-T. Xu, H. Huang
MicroRNA-142 promotes the development of nasopharyngeal carcinoma through targeting PTEN

Eur Rev Med Pharmacol Sci
Year: 2019
Vol. 23 - N. 9
Pages: 3806-3812
DOI: 10.26355/eurrev_201905_17807