OBJECTIVE: Recent studies have revealed the important role of long noncoding RNAs (lncRNAs) in regulating the progression of tumorigenesis. Oral squamous cell carcinoma (OSCC) is a prevalent tumor in the world. This study aims to identify the specific mechanism of lncRNA CASC2 in alleviating the progression of OSCC.
PATIENTS AND METHODS: CASC2 expression in OSCC cell lines and 40 paired OSCC samples were detected by quantitative Real-time polymerase chain reaction (qRT-PCR). Moreover, in vitro functions of CASC2 in regulating the cellular behaviors of OSCC cells were identified by performing transwell assay, wound healing assay and proliferation assay. The underlying mechanism of CASC2 in mediating the progression of OSCC was explored by qRT-PCR and Western blot.
RESULTS: CASC2 expression was remarkably downregulated in OSCC tissues compared with that in adjacent normal samples. Moreover, proliferation, invasion and migration were inhibited in OSCC cells overexpressing CASC2. CASC2 overexpression in OSCC cells downregulated CDK1 at both mRNA and protein levels in vitro. Besides, the expression of CDK1 in OSCC tissues was negatively correlated to the expression of CASC2.
CONCLUSIONS: CASC2 suppresses the migration, invasion and proliferation of OSCC cells through downregulating CDK1, which may offer a new therapeutic intervention for OSCC patients.
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To cite this article
H.-B. Xing, H.-M. Qiu, Y. Li, P.-F. Dong, X.-M. Zhu
Long noncoding RNA CASC2 alleviates the growth, migration and invasion of oral squamous cell carcinoma via downregulating CDK1
Eur Rev Med Pharmacol Sci
Vol. 23 - N. 11