OBJECTIVE: Recent studies have revealed that long noncoding RNAs (lncRNAs) play a crucial role in tumor progression. Melanoma is a common type of fatal cancer worldwide. The aim of this study was to identify the exact role of lncRNA SNHG7 in the progression of melanoma and to explore the possible underlying mechanism.
PATIENTS AND METHODS: SNHG7 expression in both melanoma cells and tissue samples was detected by Real-time quantitative polymerase chain reaction (RT-qPCR). The function of SNHG7 was identified by transwell assay and wound healing assay in vitro, respectively. Moreover, the underlying mechanism was explored by RT-qPCR and Western blot assay.
RESULTS: SNHG7 expression in tumor tissues was remarkably up-regulated when compared with normal tissues. The migration and invasion of melanoma cells were significantly inhibited after the silence of SNHG7 in vitro. After the silence of SNHG7, both the mRNA and protein levels of SOX4 were significantly down-regulated. Besides, the expression of SOX4 in tumor tissues was positively correlated with the expression of SNHG7.
CONCLUSIONS: Our findings suggested that SNHG7 could promote the invasion and migration of melanoma cells through up-regulating SOX4, which might offer a new therapeutic intervention for melanoma patients.
To cite this article
C. Zhang, B. Zhu, X.-B. Li, Y.-Q. Cao, J.-C. Yang, X. Li, Y.-X. Liu, Y.-B. Wang
Long non-coding RNA SNHG7 promotes migration and invasion of melanoma via upregulating SOX4
Eur Rev Med Pharmacol Sci
Vol. 23 - N. 11