Correlation between COX-2 gene polymorphism and susceptibility to nasopharyngeal carcinoma

N. Sun, X.-Q. Wang

Department of Radiotherapy, Weifang People’s Hospital, Weifang, China. 975820472@qq.com

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• Oncology

OBJECTIVE: The aim of this study was to investigate cyclooxygenase-2 (COX-2) gene polymorphism in peripheral blood cells of patients with nasopharyngeal carcinoma (NPC) and normal people, and to explore the correlation between polymorphism and the occurrence of NPC.

PATIENTS AND METHODS: The genotype and allele distributions of gene loci COX-2-899 (G/C) and COX-2-1195 (G/A) in peripheral blood of 56 normal people and 114 NPC patients were analyzed via reverse transcription-polymerase chain reaction (RT-PCR). The genetic equilibrium was detected by TaqMan genotyping technique. Meanwhile, the risk factors for NPC were analyzed via multivariate logistic regression analysis. Subsequently, the effects of risk factors, clinical features, and gene polymorphism of NPC on the prognosis and survival of patients were analyzed using univariate and multivariate COX regression analysis. Finally, the correlation of smoking, Epstein-Barr (EB) virus infection and COX-2-1195 gene polymorphism with NPC was explored via χ2-test.

RESULTS: There was a significant difference in the genotype and allele distributions at COX-2-1195 (G/A) between the two groups (p<0.05). However, no significant differences were observed at COX-2-899 (G/C) between the two groups (p>0.05). According to the results of univariate and multivariate COX regression analysis, smoking and EB virus infection were risk factors for NPC (p<0.05). The χ2-test indicated that there was an evident gene polymorphism at COX-2-1195 in smokers and EB virus-infected people compared to non-smokers and non-infected people (p<0.05).

CONCLUSIONS: COX-2-1195 gene polymorphism is associated with susceptibility to NPC. Smoking and EB virus infection are major risk factors for NPC, both of which can affect COX-2-1195 gene polymorphism.

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