Eur Rev Med Pharmacol Sci 2019; 23 (20): 9033-9040

DOI: 10.26355/eurrev_201910_19305

Correlation between endothelial cell apoptosis and SIRT3 gene expression in atherosclerosis rats

S.-H. Jing, B. Yu, H. Qiao

Department of Cardiovascular, The 2nd Affiliated Hospital of Harbin Medical University, Harbin, China. jingshenhong7915@163.com


OBJECTIVE: To investigate the correlation between the endothelial cell apoptosis and sirtuin-3 (SIRT3) gene expression in atherosclerosis (AS) rats.
MATERIALS AND METHODS: The AS model in rats was established through the high-fat diet. A total of 12 rats fed normally were enrolled as the control group, while 12 rats fed with high-fat diet were enrolled as the experimental group. After the experiment, the aortic tissues of rats were collected, and the relative area of the arterial plaque (total area of plaque/total area of the vessel) was measured via oil red O staining. The serum was collected to detect the levels of blood lipid, including total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C). Moreover, the expression levels of SIRT3 and apoptotic genes were determined via Reverse Transcription-Polymerase Chain Reaction (RT-PCR), Western blotting and immunohistochemistry (IHC), respectively. The apoptosis was detected via terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining.
RESULTS: The area of aortic plaque in the experimental group [(36.15±9.52)%] was significantly larger than that in the control group [(11.62±3.25)%] (p<0.01). Compared with those in the control group, the serum TC, TG and LDL-C levels were significantly increased in the experimental group, while the HDL-C level was significantly decreased (p<0.05). Compared with those in the control group, the mRNA and protein expression levels of SIRT3 in the aorta of rats markedly declined in the experimental group (p<0.05), while Caspase-3 and Caspase-9 expressions were significantly increased (p<0.05), respectively. The results of TUNEL staining revealed that the apoptosis in the aorta of rats in the experimental group was remarkably higher than that in the control group (p<0.05).
CONCLUSIONS: The expression of SIRT3 is deleted in the aorta of AS rats and closely related to the apoptosis. SIRT3 may serve as a potential target for the treatment of AS.

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To cite this article

S.-H. Jing, B. Yu, H. Qiao
Correlation between endothelial cell apoptosis and SIRT3 gene expression in atherosclerosis rats

Eur Rev Med Pharmacol Sci
Year: 2019
Vol. 23 - N. 20
Pages: 9033-9040
DOI: 10.26355/eurrev_201910_19305