MiRNA-300 suppresses proliferation, migration and invasion of non-small cell lung cancer via targeting ETS1

Y.-B. Yang, H. Tan, Q. Wang

Departments of Respiratory and Critical Care Medicine, Zhongnan Hospital of Wuhan University, Wuhan, China. Qiangwang1111@163.com

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• Oncology

OBJECTIVE: To elucidate the biological function of microRNA-300 (miRNA-300) in influencing the progression of non-small cell lung cancer (NSCLC).

PATIENTS AND METHODS: The relative level of miRNA-300 in NSCLC tissues and cells was determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The influences of miRNA-300 on the proliferative, migratory, and invasive potentials of A549 cells were assessed. The binding relationship between miRNA-300 and ETS1 was verified by the Dual-Luciferase Reporter Gene Assay. Finally, the regulatory effect of the interactive miRNA-300 and ETS1 on the progression of NSCLC was identified.

RESULTS: MiRNA-300 was downregulated in NSCLC tissues and cells. The overexpression of miRNA-300 suppressed A549 cells to proliferate, migrate, and invade. MiRNA-300 could bind to ETS1 3’UTR and suppress its level in A549 cells. Notably, the knockdown of miRNA-300 could reverse the regulatory role of ETS1 in the cellular behaviors of NSCLC.

CONCLUSIONS: MiRNA-300 suppresses NSCLC to proliferate, migrate, and invade by targeting ETS1.

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