Eur Rev Med Pharmacol Sci 2020; 24 (3): 1454-1459

DOI: 10.26355/eurrev_202002_20204

In vitro effect of some nucleoside reverse transcriptase inhibitors against HSV-1 replication

R. Nahid Samiei, S. Ebrahimi, M. Fani, S. Ghafari, M. Makvandi, N. Khodadad, M. Tabasi, A. Teimoori

Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. teimooriali1982@gmail.com


OBJECTIVE: The current study was designed to investigate the effects of some nucleoside reverse transcriptase inhibitors (NRTIs) on HSV-1 infection.

MATERIALS AND METHODS: Initially, the SwissTargetPrediction server was used to predict the interactions between HSV-1 thymidine kinase and acyclovir, stavudine, zidovudine, didanosine, and entecavir. The effect of each component on Vero cell viability was assessed by the MTT assay. After treatment, the cell supernatants were collected, and HSV-1 replication was analyzed by quantitative real-time PCR.

RESULTS: The qPCR results revealed that viral titers were reduced 41, 40, 19, 44, and 31-fold in the presence of acyclovir, zidovudine, stavudine, didanosine, and entecavir, respectively.

CONCLUSIONS: Our findings indicate that NRTIs significantly reduce HSV-1 replication in cell culture.

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To cite this article

R. Nahid Samiei, S. Ebrahimi, M. Fani, S. Ghafari, M. Makvandi, N. Khodadad, M. Tabasi, A. Teimoori
In vitro effect of some nucleoside reverse transcriptase inhibitors against HSV-1 replication

Eur Rev Med Pharmacol Sci
Year: 2020
Vol. 24 - N. 3
Pages: 1454-1459
DOI: 10.26355/eurrev_202002_20204