Eur Rev Med Pharmacol Sci 2020; 24 (5): 2248-2255

DOI: 10.26355/eurrev_202003_20490

LncRNA MIF-AS1 aggravates the progression of ovarian cancer by sponging miRNA-31-5p

Y. Fan, L. Wang, X.-C. Han, H.-Y. Ma, N. Zhang, L. Zhe

Department of Gynecology, People’s Hospital of Ningxia Hui Autonomous Region, Yinchuan, China. yangf0803@126.com


OBJECTIVE: The aim of this study was to uncover the role of lncRNA MIF-AS1 in influencing the biological phenotypes of ovarian cancer (OC) and the underlying mechanism.

PATIENTS AND METHODS: OC tissues and adjacent normal tissues were collected from 50 OC patients. The expression level of lncRNA MIF-AS1 in OC tissues and cells was determined by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR). The prognostic potential of MIF-AS1 in OC patients was assessed by the Kaplan-Meier method. Subsequently, the regulatory effects of MIF-AS1 on proliferative, migratory, and invasive abilities of ES-2 and HO-8910 cells were evaluated by a series of functional experiments. Dual-Luciferase reporter gene assay, qRT-PCR, and Western blot were further conducted to verify the interaction in the regulatory loop MIF-AS1/miRNA-31-5p/PLCB1.

RESULTS: MIF-AS1 was significantly upregulated in OC tissues and cell lines (p<0.05). Higher level of MIF-AS1 predicted significantly worse prognosis of OC patients (p<0.05). The knockdown of MIF-AS1 markedly attenuated the proliferative, migratory, and invasive abilities of ES-2 and HO-8910 cells (p<0.05). Dual-Luciferase reporter gene assay verified that MIF-AS1 competed with PLCB1 to bind miRNA-31-5p. In addition, MIF-AS1 negatively regulated miRNA-31-5p expression cells, and miRNA-31-5p negatively regulated PLCB1 expression in OC.

CONCLUSIONS: MIF-AS1 was significantly upregulated in OC, which accelerated the proliferative, migratory, and invasive abilities of OC cells. Furthermore, the regulatory loop MIF-AS1/miRNA-31-5p/PLCB1 could be utilized as a therapeutic target for OC.

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To cite this article

Y. Fan, L. Wang, X.-C. Han, H.-Y. Ma, N. Zhang, L. Zhe
LncRNA MIF-AS1 aggravates the progression of ovarian cancer by sponging miRNA-31-5p

Eur Rev Med Pharmacol Sci
Year: 2020
Vol. 24 - N. 5
Pages: 2248-2255
DOI: 10.26355/eurrev_202003_20490