MicroRNA-150-5p inhibits proliferation and invasion of osteosarcoma cells by down-regulating VEGFA

Y. Qin, B. Zhang, B.-J. Ge

Department of Orthopedics, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. 1297964958@qq.com

---

• Oncology

OBJECTIVE:  The aim of this study was to investigate whether microRNA-150-5p was involved in osteosarcoma cell proliferation and invasiveness via modulating vascular endothelial growth factor A (VEGFA) expression.

PATIENTS AND METHODS: 10 pairs of osteosarcoma tissues and para-cancerous tissues were collected from patients with osteosarcoma in our center from February 2012 to July 2018. Relative expression levels of microRNA-150-5p and VEGFA in tissues and cell lines were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Luciferase reporter gene assay was conducted to illustrate the binding interplay between microRNA-150-5p and VEGFA. Furthermore, proliferative and invasive potentials in HOS and MG-63 cells regulated by both microRNA-150-5p and VEGFA were determined using Cell Counting Kit-8 (CCK-8), colony formation assay, and transwell assay, respectively.

RESULTS: MicroRNA-150-5p was remarkably downregulated, while VEGFA was upregulated in osteosarcoma tissues compared with para-cancerous tissues (p<0.05). Similar results were observed in osteosarcoma cells and normal osteoblasts. Overexpression of microRNA-150-5p significantly inhibited the proliferation and invasion of osteosarcoma cells (p<0.05). Luciferase reporter gene assay demonstrated that microRNA-150-5p could target to VEGFA to negatively modulate its expression. In addition, the knockdown of VEGFA remarkably weakened osteosarcoma cell proliferative and invasive capacities (p<0.05).

CONCLUSIONS: MicroRNA-150-5p weakens proliferative and invasive potentials in osteosarcoma cells by downregulating VEGFA level. All our findings suggest that microRNA-150-5p/VEGFA axis is a promising target for osteosarcoma treatment.

Free PDF Download