Eur Rev Med Pharmacol Sci 2020; 24 (21): 11365-11373

DOI: 10.26355/eurrev_202011_23628

Beneficial effect of oral administration of zinc sulfate on 5-fluorouracil-induced gastrointestinal mucositis in rats

C. Tefas, L. Ciobanu, C. Berce, A. Meșter, S. Onica, C. Toma, M. Tanțău, M. Taulescu

Iuliu Hațieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania. ciobanulidia@yahoo.com


OBJECTIVE: This experimental study explored the potential of oral zinc sulfate to protect the gut mucosa from 5-fluorouracil (5-FU)-induced degenerative lesions in Wistar rats.

MATERIALS AND METHODS: Female Wistar rats were used and divided into 2 interventional groups (Z with 6 animals and F with 5 animals) and one control group (M with 5 rats). After 2 hours of fasting, group Z received via oral gavage 1.5 ml of solution, corresponding to 15 mg zinc sulfate for 9 consecutive days. Groups F and M received only the vehicles. On day 3, 400 mg/kg of 5-FU was administered intraperitoneally to groups Z and F. Tissue samples were collected from the duodenum, jejunum, colon and liver. Histological assessment for each gastrointestinal tract segment was determined semi-quantitatively by rating 11 histological features from normal (0) to severe (3). The independent groups were analyzed using the Kruskal-Wallis test and the Mann-Whitney U-test, with a Bonferroni correction for alpha (p ≤ 0.016).

RESULTS: In group F the jejunum was the most affected area with a mean histological score of  27 (25-32). In the Z group, significantly lower histological scores were obtained compared with group F (duodenum Z vs. F: U = 0, p = 0.004; jejunum Z vs. F: U = 0, p = 0.006 and colon: Z vs. F: U = 0, p = 0.005). Graded liver necro-inflammatory lesions were significantly lower in group Z compared with group F (U = 0, p = 0.004), suggesting fewer bacterial intestinal translocation processes.

CONCLUSIONS: Zinc sulfate has a beneficial role, decreasing the severity of gut mucosal injuries induced by 5-FU in Wistar rats.

 

Free PDF Download

To cite this article

C. Tefas, L. Ciobanu, C. Berce, A. Meșter, S. Onica, C. Toma, M. Tanțău, M. Taulescu
Beneficial effect of oral administration of zinc sulfate on 5-fluorouracil-induced gastrointestinal mucositis in rats

Eur Rev Med Pharmacol Sci
Year: 2020
Vol. 24 - N. 21
Pages: 11365-11373
DOI: 10.26355/eurrev_202011_23628