LncRNA PSMA3-AS1 promotes colorectal cancer cell migration and invasion via regulating miR-4429

P. Peng, Y. Wang, B.-L. Wang, Y.-H. Song, Y. Fang, H. Ji, C.-N. Huangfu, K.-M. Wang, Q. Zheng

The Second Clinical Medical College of Nanjing Medical University, Nanjing, Jiangsu, China. kemingwang@sahnum.cn

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• Oncology

OBJECTIVE: Many studies have revealed that long non-coding RNAs (lncRNAs) are related to various cancers, including colorectal cancer (CRC). This study aims to explore the biological function of lncRNA PSMA3-AS1 in CRC progression.

MATERIALS AND METHODS: The expression levels of PSMA3-AS1 and miR-4429 were assessed by RT-qPCR. CRC progression was explored by cell viability, migration, and invasion using CCK-8 and transwell assays. The interaction between PSMA3-AS1 and miR-4429 was verified by bioinformatics analysis, Dual-Luciferase assay, and RIP assay.

RESULTS: It was found that PSMA3-AS1 expression was increased and miR-4429 expression was decreased in CRC tissues and cells. In addition, PSMA3-AS1 interference markedly hindered the proliferation, migration, and invasion of CRC cells. MiR-4429 was a direct target of PSMA3-AS1, and the knockdown of PSMA3-AS1 significantly suppressed miR-4429 expression. The depletion of PSMA3-AS1 inhibited CRC progression, which was neutralized by miR-4429 inhibitor.

CONCLUSIONS: PSMA3-AS1 accelerated CRC progression by regulating miR-4429 expression, which could be used as a potential therapeutic target for CRC patients.

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