OBJECTIVE: Real-time reverse transcription-polymerase chain reaction (RT-PCR) is the gold standard examination to confirm coronavirus disease 2019 (COVID-19). This study aimed to determine the association between RT-PCR Ct value and COVID-19 clinical severity in the second week of illness.
PATIENTS AND METHODS: This study was a cross-sectional study. Data were collected from medical records of COVID-19 patients at the tertiary COVID-19 referral hospital of West Java, Dr. Hasan Sadikin Hospital Bandung, from January to May 2021. A total of 207 patients who met inclusion criteria were divided into four severity groups. The data were analyzed with One Way ANOVA Test.
RESULTS: There was no significant difference in RT-PCR Ct value among mild, moderate, severe and critical groups measured in the second week of illness, with p=0.825 for Ct Helicase/ORF1b gene, p=0.821 for Ct RdRp gene and p=0.870 for the lowest Ct gene.
CONCLUSIONS: Although Ct value reflects viral load, its role is concluded to be clinically insignificant in terms of association with the severity of COVID-19 in the second week of illness.Free PDF Download
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To cite this article
A. Yuwono Soeroto, R. Antartika, N. Nauli Asriputri, H. Suryadinata, B. Andriyoko
Real-time RT-PCR Ct value is not associated with COVID-19 disease severity: an observational study in tertiary COVID-19 referral hospital of West Java, Indonesia
Eur Rev Med Pharmacol Sci
Vol. 26 - N. 13