Background/Aims: Cellular immunity has a pivotal role in the pathogenesis of chronic pancreatitis (CP), resulting in pancreas infiltration by T-cells. Studies on systemic immunity are few and contradictory. One study reported a decrease of naive CD45RA+ cells. The presence of naive T cells, detected as recent thymic emigrants (RTEs), is evaluated with a new molecular technique by using real-time PCR to detect the T-cell receptor excision circles (TREC). To elucidate the role of naive T-cells in the pathogenesis of CP, we investigated the percentage of sj-TREC in CP patients.
Patients: Thirty CP patients were studied and compared to 30 sex- and age-matched healthy volunteers.
Methods: Genomic DNA was isolated from peripheral blood mononuclear cells (PBMC) of each patient. RTEs were evaluated by measuring sj-TREC by real-time PCR.
Results: The mean percentage of sj-TREC+ cells present in CP was not significantly different from that of control group (0.02319% vs 0.02338%, respectively).
Conclusion: Our data show that naive TREC+ cells are normally represented in CP. The presence of active thymopoiesis may be the underlying mechanism resulting in continuous production of T-cells, responsible of maintaining the inflammatory process.
Corresponding Author: Franco Pandolfi, MD; e-mail: email@example.comFree PDF Download
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R. Cianci 1, C. Spada 2, V. Perri 2, G. Cammarota 1, R. Urgesi 2, S. Lolli 1, E. Girardi 3, G. Costamagna 2, F. Pandolfi 1
Active thymopoiesis in idiopathic chronic pancreatitis
Eur Rev Med Pharmacol Sci
Vol. 12 - N. 1