Background. During intestinal inflammation white blood cells are recruited from the blood, and they represent the major contributors to tissue perpetuation of inflammation via their production of chemokines and pro-inflammatory cytokines.
Objectives. Investigate the effect of a symbiotic formulation containing Lactobacillus Paracasei B 20160 versus placebo, on serum levels of interleukin (IL)-6, tumor necrosis factor (TNF)α, IL-8, IL-1β and IL-10 and on mRNA lymphomonocyte expression of TNFα, IL-8 and IL-1β in patients with ulcerative colitis.
Materials and Methods. Eighteen patients entered the study with histologically proven not complicated ulcerative colitis, treated with mesalazine. Patients were treated for 8 weeks (9 with symbiotic and 9 with placebo). Serum levels of IL-6, TNFα, IL-8, IL-1β and IL-10 were measured using a commercially available sandwich ELISA kit. RT-PCR analysis was performed on total RNA isolated from peripheral lymphomonocytes.
Results. In basal condition, there was an increase of serum levels of TNFα, IL-6, and IL-8. The treatment with symbiotic significantly decreased serum levels of the last two cytokines (IL-6 and IL-8). In lymphocytes, the treatment with the symbiotic don’t significantly reduced the mRNA expression of TNFα and IL-1β, while that of IL-8 was strongly and significantly decreased.
Conclusions. Our preliminary results suggest that a symbiotic formulation containing Lactobacillus paracasei significantly improves the plasma and lymphocyte content of some proinflammatory cytokines.
Corresponding Author: Alessandro Federico, MD; e-mail: email@example.comFree PDF Download
To cite this article
A. Federico, C. Tuccillo, E. Grossi*, R. Abbiati*, N. Garbagna*, M. Romano, A. Tiso, C. Del Vecchio Blanco, C. Loguercio
The effect of a new symbiotic formulation on plasma levels and peripheral blood mononuclear cell expression of some pro-inflammatory cytokines in patients with ulcerative colitis: a pilot study
Eur Rev Med Pharmacol Sci
Vol. 13 - N. 4