Evaluation of cytotoxicity of some common ophthalmic drugs

M. Li, X.-M. Chen, J.-J. Liu, D.-M. Wang, L. Gan, X. Lv, Y. Qiao

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• Ophthalmology

OBJECTIVE: The study was aimed at evaluating the in vitro cytotoxicity of some commonly used drugs in ophthalmology. Hydrocortisone Sodium Succinate, Dexamethasone Sodium Phosphate, 5-Fluorouracil, Tobramycin and Pilocarpine Nitrate are frequently used in various indications involving eye care, and the aim was to test the safety of these in cell culture.

MATERIALS AND METHODS: The in vitro cytotoxicity was carried out on the NIH 3T3 cell line by the Sulforhodamine B (SRB) assay.

RESULTS: With the exception of 5-Fluorouracil, none of the other drugs demonstrated appreciable cytotoxicity up to high concentrations of 200 µg/ml at 48 hours of drug exposure.

CONCLUSIONS: Hydrocortisone Sodium Succinate, Dexamethasone Sodium Phosphate, Tobramycin and Pilocarpine Nitrate were confirmed to be non-cytotoxic while 5-Fluorouracil was highly cytotoxic especially at 48 hours at very low concentrations.

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