MiR-324-5p inhibits proliferation of glioma by target regulation of GLI1

H.-s. Xu, H.-l. Zong, M. Shang, X. Ming, J.-p. Zhao, C. Ma, L. Cao

Department of Neurosurgery, the Central Hospital of Xuzhou, Xuzhou Clinical School of Xuzhou Medical College, Affiliated Hospital of Southeast University, Xuzhou, Jiangsu, People’s Republic of China. xhs51@126.com

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• Oncology

OBJECTIVES: To study the effects of the miR-324-5p on the glioma cells proliferation via the targeted regulation of the glioma-associated oncogene 1.

METHODS: The luciferase reporter gene was used to test whether the glioma-associated oncogene 1 was the target of the miR-324-5p microRNA. The glioma-associated oncogene 1 expression was detected by Western blot. The proliferation and cell cycle were evaluated by MTT assay and flow cytometry.

RESULTS: The glioma-associated oncogene 1 is a target of the miR-324-5p. An over-expressed miR-324-5p could reduce the cell survival rate and increase the G1/G0 phase rate in the glioma cell lines.

CONCLUSIONS: The miR-324-5p can inhibit proliferation of the glioma cells via the targeted regulation of the glioma-associated oncogene 1.

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