Id1 knockdown induces the apoptosis and inhibits the proliferation and invasion of ovarian cancer cells

W.-Z. Sun, M.-H. Li, M. Chu, L.-L. Wei, M.-Y. Bi, Y. He, L.-B. Yu

Department of Gynecology, The Affiliated Tumor Hospital of Harbin Medical University, Harbin, China. sjystudent@126.com

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• Oncology

OBJECTIVE: To explore the role of Id1 in ovarian cancer cell proliferation, invasion and apoptosis.

MATERIALS AND METHODS: Lentivirus-based shRNA vectors were constructed to knockdown Id1 expression in SKOV3 ovarian cancer cells. The proliferation, invasion ability and apoptosis of SKOV3 cells were evaluated by CCK-8 assay, transwell assay and flow cytometry, respectively.

RESULTS: Compared to control cells, cell proliferation and invasion were significantly inhibited in SKOV3 cells depleted of Id1, while apoptosis was significantly increased in SKOV3 cells depleted of Id1.

CONCLUSIONS: Id1 functions to promote ovarian cancer cell proliferation and invasion, and Id1 is a promising therapeutic target for ovarian cancer.

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