Eur Rev Med Pharmacol Sci 2018; 22 (6): 1819-1824

DOI: 10.26355/eurrev_201803_14601

Butorphanol attenuates myocardial ischemia reperfusion injury through inhibiting mitochondria-mediated apoptosis in mice

L.-H. Huang, J. Li, J.-P. Gu, M.-X. Qu, J. Yu, Z.-Y. Wang

Heart Function Examination Room, The Second Affiliated Hospital of Dalian Medical University, Dalian, China. yujian862@sina.com


OBJECTIVE: To investigate the role of the opioid receptors agonist butorphanol on mice myocardial ischemia reperfusion (I/R) injury.

MATERIALS AND METHODS: The left anterior descending of coronary artery was ligatured for 30 min and then reperfusion for 6 h was performed to mimic the mouse myocardial I/R injury. All mice were randomly divided into three groups: sham group, I/R group and I/R + butorphanol group. Blood samples were collected for the measurement of cardiac troponin I (CTnI) and creatine kinase MB (CK-MB) levels. The infarct size was stained by triphenyltetrazolium chloride. The mitochondria morphology was observed by electron microscopy. The expressions of cleaved caspase-9 and -3, p38, ERK and JNK were detected by Western blot.

RESULTS: The myocardial infarct size, serum CK-MB and CTn I levels, expression of cleaved caspase-9 and -3, phosphorylation of p38 and JNK were all increased in the I/R group compared with the sham group (all p < 0.01). Butorphanol reduced the myocardial infarct size, serum CTn I and CK-MB levels, expression of cleaved caspase-9 and -3, and phosphorylation levels of p38 and JNK (all p < 0.01). The number of mitochondria and the individual mitochondrial cross-sectional areas were decreased in the I/R mice compared with the sham-operated mice (all p < 0.01). Butorphanol reversed these changes in mitochondrial morphology (all p < 0.01).

CONCLUSIONS: Butorphanol attenuates myocardial I/R injury through reducing cardiomyocyte apoptosis by inhibiting mitochondria-mediated apoptotic pathway, and blockage of p38 and JNK phosphorylation.

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To cite this article

L.-H. Huang, J. Li, J.-P. Gu, M.-X. Qu, J. Yu, Z.-Y. Wang
Butorphanol attenuates myocardial ischemia reperfusion injury through inhibiting mitochondria-mediated apoptosis in mice

Eur Rev Med Pharmacol Sci
Year: 2018
Vol. 22 - N. 6
Pages: 1819-1824
DOI: 10.26355/eurrev_201803_14601