Eur Rev Med Pharmacol Sci 2019; 23 (20): 8833-8840

DOI: 10.26355/eurrev_201910_19278

MiR-203 regulates DJ-1 expression and affects proliferation, apoptosis and DDP resistance of pancreatic cancer cells

S.-L. Du, L.-Y. Xu, P. Gao, Q.-S. Liu, F.-F. Lu, Z.-H. Mo, Z.-Z. Fan, X.-L. Cheng, Z.-H. Dong

Department of Medical Imaging, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan, China. zhuangrenzhong9jr@yeah.net


OBJECTIVE: DJ-1 is a negative regulator of PTEN and plays a role in tumorigenesis. Abnormal miR-203 expression is associated with pancreatic cancer. Bioinformatics analysis showed a targeted relationship between miR-203 and DJ-1 3’-UTR. This study investigated whether miR-203 regulates DJ-1 expression and its role in pancreatic cancer cell proliferation, apoptosis, and cisplatin (DDP) resistance.
MATERIALS AND METHODS: The Dual-Luciferase reporter gene assay validated the targeted regulation between miR-203 and DJ-1. The DDP-resistant cell line SW1990/DDP was established and divided into miR-NC group and miR-203 mimic group followed by analysis of the expression of DJ-1, PTEN and p-AKT, cell apoptosis, and proliferation.
RESULTS: There was a targeted relationship between miR-203 and DJ-1 mRNA. The expression of miR-203 in SW1990/DDP cells was significantly lower than that in SW1990 cells, while the expression of DJ-1 mRNA and protein was significantly higher than that in SW1990 cells. Compared with miR-NC group, the expression of DJ-1 and p-AKT protein in SW1990/DDP cells was significantly decreased in miR-203 mimic transfection group, while the expression of PTEN was significantly increased with increased cell apoptosis and decreased cell proliferation, as well as reduced DDP resistance.
CONCLUSIONS: The decreased expression of miR-203 and the increased expression of DJ-1 is associated with drug resistance in pancreatic cancer cells. Elevated miR-203 can inhibit the expression of DJ-1, affect the activity of PTEN-PI3K/AKT pathway, inhibit the proliferation of pancreatic cancer cells, induce cell apoptosis, and reduce DDP resistance of pancreatic cancer cells.

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S.-L. Du, L.-Y. Xu, P. Gao, Q.-S. Liu, F.-F. Lu, Z.-H. Mo, Z.-Z. Fan, X.-L. Cheng, Z.-H. Dong
MiR-203 regulates DJ-1 expression and affects proliferation, apoptosis and DDP resistance of pancreatic cancer cells

Eur Rev Med Pharmacol Sci
Year: 2019
Vol. 23 - N. 20
Pages: 8833-8840
DOI: 10.26355/eurrev_201910_19278