OBJECTIVE: Recently, long non-coding RNAs (lncRNAs) have been widely studied for their vital roles in human diseases. In this study, we investigated the effect of lncRNA AB073614 on the metastasis of gastric cancer (GC), and explored the possible underlying mechanism.
PATIENTS AND METHODS: AB073614 expression in GC tissue samples was detected by Real-time quantitative polymerase chain reaction (RT-qPCR). The roles of AB073614 in GC metastasis were identified through wound healing assay and transwell assay, respectively. Moreover, RT-qPCR and Western blot assay were used to explore the potential mechanism.
RESULTS: AB073614 expression level in GC samples was significantly higher than that of adjacent ones. Besides, the migration and invasion of GC cells were obviously repressed after AB073614 was knocked down. After AB073614 was knocked down in vitro, the mRNA and protein expressions of insulin-like growth factor 2 (IGF-2) was remarkably down-regulated. Furthermore, a negative correlation was found between the expression level of IGF-2 and AB073614 in GC tissues.
CONCLUSIONS: AB073614 could promote GC cell migration and invasion via up-regulating IGF-2. Our findings might provide a potential therapeutic target for GC patients.Free PDF Download
To cite this article
X.-Y. Wu, H.-Y. Zhou, X.-M. Yao, X.-D. Chen, J. Wu, X.-C. Lu
Long non-coding RNA AB073614 promotes metastasis of gastric cancer cells by upregulating IGF-2
Eur Rev Med Pharmacol Sci
Vol. 24 - N. 1