Eur Rev Med Pharmacol Sci 2020; 24 (5): 2616-2624

DOI: 10.26355/eurrev_202003_20530

Long non-coding RNA MIR4435-2HG recruits miR-802 from FLOT2 to promote melanoma progression

D.-M. Ma, D. Sun, J. Wang, D.-H. Jin, Y. Li, Y.-E. Han

Department of Dermatology and Venereology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province, China. gongicdnxm265@163.com


OBJECTIVE: The regulatory mechanism of lncRNA MIR4435-2HG has been extensively investigated in human cancers other than melanoma. This study aims to elucidate the role of lncRNA MIR4435-2HG in melanoma.

MATERIAL AND METHODS: The mRNA expression was detected by RT-qPCR. MTT assay, Transwell assay and Dual-Luciferase reporter assay were used to investigate the regulatory mechanism of lncRNA MIR4435-2HG.

RESULTS: Upregulation of lncRNA MIR4435-2HG was identified in melanoma and promoted melanoma cell proliferation, migration and invasion. In addition, lncRNA MIR4435-2HG serves as the ceRNA of miR-802. MiR-802 inhibited melanoma progression by downregulating lncRNA MIR4435-2HG. Besides, miR-802 directly targets FLOT2. And knockdown of FLOT2 restrained the progression of melanoma by downregulating lncRNA MIR4435-2HG and upregulating miR-802.

CONCLUSIONS: LncRNA MIR4435-2HG promotes cell proliferation, migration and invasion in melanoma by sponging miR-802 and upregulating FLOT2.

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To cite this article

D.-M. Ma, D. Sun, J. Wang, D.-H. Jin, Y. Li, Y.-E. Han
Long non-coding RNA MIR4435-2HG recruits miR-802 from FLOT2 to promote melanoma progression

Eur Rev Med Pharmacol Sci
Year: 2020
Vol. 24 - N. 5
Pages: 2616-2624
DOI: 10.26355/eurrev_202003_20530