Eur Rev Med Pharmacol Sci 2022; 26 (9): 3301-3309

DOI: 10.26355/eurrev_202205_28749

Acyl-CoA binding protein (ACBP) is highly expressed in the developing human kidney

M. Piras, C. Gerosa, D. Fanni, F. Cau, P. Coni, R. Murru, G. Denotti, G. OrrĂ¹, A. Scano, F. Ledda, P. Van Eyken, F. Coghe, G. Faa, M. Castagnola

Department of Medical Sciences and Public Health, University of Cagliari, Electron Microscopy Laboratory, Division Pathological Anatomy, Cagliari, Italy. monica.piras@unica.it


OBJECTIVE: Acyl-CoA-binding protein (ACBP), also known as diazepam binding inhibitor (DBI), is a small phylogenetically conserved protein. This ancestral peptide is multifunctional, performing intracellular activities as ACBP protein or extracellular roles as DBI. Several studies showed its endless facets, including a relevant activity as appetite stimulator and as anabolic factor. High levels of ACBP have been described in erythrocytes, liver, kidney, and gut cells. The aim of this study was to analyze, at immunohistochemical level, the expression of ACBP in fetal human tissues during development, focusing on the developing kidney.

MATERIALS AND METHODS: Immunohistochemistry for ACBP was performed on 30 human fetal kidneys, from 15 fetuses of gestational age ranging from 13 to 19 weeks. At autopsy, all kidney samples were 10% formalin-fixed, routinely processed and paraffin-embedded. Five micron-thick paraffin sections were stained with Hematoxylin and Eosin and PAS stain for a morphological examination.

RESULTS: ACBP was detected in all 30 kidneys analyzed in this study. No significant changes in ACBP expression were observed at different gestational ages. Immunostaining for ACBP was restricted to the epithelium covering the renal pelvis, the papillae, the collecting tubules, and the proximal and distal tubules. On the other hand, medullary regions and in the metanephric mesenchymal stem/progenitor cells did not show any reactivity for ACBP.

CONCLUSIONS: According to our findings, ACBP should be considered as a new player in the complex field of human nephrogenesis, given that it was detected in all fetal kidneys immunostained. Its preferential localization in the renal structures derived from the Wolf duct, such as pelvis epithelium and collecting ducts, suggests a major role for ACBP in the induction of the metanephric mesenchymal cells toward the differentiation into glomerular structures. ACBP expression in proximal and distal tubules, two structures originating from the metanephric mesenchyme, indicates a further role of this protein in nephron development. In conclusion, ACBP should be added to the multiple molecules involved in human nephrogenesis.

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M. Piras, C. Gerosa, D. Fanni, F. Cau, P. Coni, R. Murru, G. Denotti, G. OrrĂ¹, A. Scano, F. Ledda, P. Van Eyken, F. Coghe, G. Faa, M. Castagnola
Acyl-CoA binding protein (ACBP) is highly expressed in the developing human kidney

Eur Rev Med Pharmacol Sci
Year: 2022
Vol. 26 - N. 9
Pages: 3301-3309
DOI: 10.26355/eurrev_202205_28749